Distribution of Bulk and HIV-specific CD8 + T Cell Memory Phenotypes during Acute/Early HIV Infection Is Related to Reduced Antiviral Activity

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dc.contributor.authorGhiglione, Yanina
dc.contributor.authorFalivene, Juliana
dc.contributor.authorRuiz, Maria
dc.contributor.authorLaufer, Natalia
dc.contributor.authorSocias, Maria E.
dc.contributor.authorCahn, Pedro
dc.contributor.authorSued, Omar
dc.contributor.authorSalomon, Horacio
dc.contributor.authorGherardi, María Magdalena
dc.contributor.authorTurk, Gabriela
dc.date.accessioned2024-05-23T15:24:12Z
dc.date.available2024-05-23T15:24:12Z
dc.date.issued2014
dc.description.abstractBackground: Memory CD8+ T-cells are important components of protective immunity. Understanding their development during primary HIV infection (PHI) may contribute to optimal vaccine design. Aim: To analyze the distribution of memory subsets during PHI and their correlation with functionality and clinical parameters. Methods: 19 samples from acutely infected subjects were obtained at baseline and 12 months post-infection (mpi). Phenotypic (CD45RO, CCR7, PD-1) and functional markers (cytokines) were used to identify bulk and HIV-specific CD8+ memory populations. CD8 virus inhibitory assay (VIA) was performed. Data was compared intra-group and correlated to clinical parameters, PD-1 analysis and CD8 antiviral activity, using non-parametric statistics. Results: Bulk and HIV-specific CD8+ profile was terminal effectors (TE)>naïve>effector memory (TEM)>central memory. Spearman's correlation showed that baseline CD8+ VIA inversely correlated with the concurrent proportion of HIV-specific CD8+ TEM cells (r=-0.593, p=0.009) and directly correlated with the proportion of HIV-specific CD8+ TE cells (r=0.718, p=0.0008). Identical correlations were observed between baseline CD8+ T cell phenotype and CD8+ VIA at 12 mpi. Also, percentage of PD-1high CD8+ T cells negatively correlated with bulk and HIV-specific CD8+ TEM cells (r=−0.501, p=0.034 and r=−0.668, p=0.004, respectively). Conversely, positive correlations were observed with the proportion of bulk and HIV-specific CD8+ TE cells (r=-0.510, p=0.0308 and r=−0.564, p=0.022, respectively). Conclusions: A higher proportion of fully differentiated HIV-specific cells are related to the magnitude of CD8+ antiviral activity (rapidly able to exert effector functions) and to a higher PD-1 expression (related to T cell differentiation stage and activation status). This is the first report were a relation between CD8+ T cell memory differentiation hierarchy and antiviral function is reported during acute infection, providing information potentially useful for vaccine design.
dc.identifier.citationGhiglione, Y., Falivene, J., Ruiz, M. J., Laufer, N., Socias, M. E., Cahn, P., ... & Turk, G. (2014). Distribution of bulk and HIV-specific CD8+ T cell memory phenotypes during acute/early HIV infection is related to reduced antiviral activity. AIDS Research and Human Retroviruses, 30(S1), A179-A179.
dc.identifier.other10.1089/aid.2014.5380.abstract
dc.identifier.urihttps://repositorio.huesped.org.ar/handle/123456789/1056
dc.relation.ispartofseriesAIDS Research and Human Retroviruses, 30(S1)
dc.subjectCD8+ T cells
dc.subjectHIV-specific memory
dc.subjectAntiviral activity
dc.titleDistribution of Bulk and HIV-specific CD8 + T Cell Memory Phenotypes during Acute/Early HIV Infection Is Related to Reduced Antiviral Activity

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